1. What is Relative Centrifugal Force (RCF)?

Definition: RCF, or g-force, is the measurement of the acceleration applied to a sample in a centrifuge.

Purpose: It helps scientists and lab technicians standardize centrifugation protocols across different equipment.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( RCF \) — Relative centrifugal force (g-force)
• \( RPM \) — Rotations per minute
• \( r \) — Rotor radius in centimeters

Explanation: The formula relates the rotational speed (RPM) and rotor radius to the actual force experienced by the sample.

3. Importance of RCF Calculation

Details: Proper RCF calculation ensures reproducible results across different centrifuges and maintains sample integrity.

4. Using the Calculator

Tips: Enter the centrifuge speed in RPM and the rotor radius in centimeters. All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of just RPM?
A: RCF accounts for rotor radius, making protocols transferable between different centrifuges.

Q2: How do I measure rotor radius?
A: Measure from the center of the rotor to the bottom of the sample holder when it's in horizontal position.

Q3: What's a typical RCF range?
A: Common ranges are 1,000-15,000g for routine work, up to 100,000g for ultracentrifugation.

Q4: Why is the constant 894 used?
A: It comes from the gravitational acceleration constant (981 cm/s²) converted to appropriate units.

Q5: Can I calculate RPM from RCF?
A: Yes, with the formula: \( RPM = \sqrt{\frac{RCF \times 894}{r}} \)