Centrifuge Rpm To G Calculator

Relative Centrifugal Force (RCF) Formula:

\[ g = \frac{RPM^2 \times r}{894} \]

Relative Centrifugal Force (g):

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1. What is a Centrifuge RPM to G Calculator?

Definition: This calculator converts centrifuge rotational speed (RPM) to relative centrifugal force (RCF or g-force) based on the rotor radius.

Purpose: It helps laboratory technicians and researchers determine the actual force applied during centrifugation.

2. How Does the Calculator Work?

The calculator uses the formula:

\[ g = \frac{RPM^2 \times r}{894} \]

Where:

\( g \) — Relative centrifugal force (g-force)
\( RPM \) — Rotations per minute
\( r \) — Rotor radius (cm)
894 — Conversion factor (derived from gravitational acceleration and unit conversions)

Explanation: The g-force increases with the square of the RPM and linearly with the rotor radius.

3. Importance of RCF Calculation

Details: Many protocols specify separation conditions in g-force rather than RPM, as g-force is what actually affects the samples.

4. Using the Calculator

Tips: Enter the centrifuge speed in RPM and the rotor radius in centimeters (measured from center to sample). All values must be > 0.

5. Frequently Asked Questions (FAQ)

Q1: Why use g-force instead of RPM?
A: G-force accounts for differences in rotor sizes - the same RPM produces different forces in different centrifuges.

Q2: How do I measure rotor radius?
A: Measure from the center of the rotor to the middle of the sample holder when it's in the horizontal position.

Q3: What's a typical g-force for common applications?
A: Routine spins: 300-500g, cell pelleting: 1000-2000g, microorganism pelleting: 5000-15000g.

Q4: Why is the relationship squared?
A: Centrifugal force depends on angular velocity squared (ω²), and RPM is proportional to ω.

Q5: Can I use this for ultracentrifuges?
A: Yes, the formula applies to all centrifuges, though ultracentrifuges typically operate at much higher g-forces.