1. What is Centrifuge RPM to G Calculation?

Definition: This calculator converts centrifuge rotational speed (RPM) to relative centrifugal force (RCF or g-force) based on the rotor radius.

Purpose: It helps laboratory technicians and researchers determine the appropriate centrifugation parameters for their experiments.

2. How Does the Calculator Work?

The calculator uses the formula:

Where:

• \( g \) — Relative centrifugal force (g-force)
• \( RPM \) — Rotations per minute
• \( r \) — Rotor radius (cm)
• 894 — Conversion factor incorporating gravitational acceleration

Explanation: The g-force increases with the square of the RPM and linearly with the rotor radius.

3. Importance of RCF Calculation

Details: Proper RCF calculation ensures consistent experimental conditions across different centrifuges and is critical for protocols specifying separation by g-force rather than RPM.

4. Using the Calculator

Tips: Enter the centrifuge speed in RPM and the rotor radius in centimeters. Measure radius from the center axis to the sample position.

5. Frequently Asked Questions (FAQ)

Q1: Why convert RPM to g-force?
A: g-force is the actual force applied to samples, while RPM alone doesn't account for rotor size differences.

Q2: How do I measure rotor radius?
A: Measure from the center of the rotor to the middle of the sample holder when it's in the horizontal position.

Q3: Why does the formula use 894?
A: This constant incorporates the gravitational acceleration (980.665 cm/s²) and unit conversions.

Q4: Can I use this for any centrifuge?
A: Yes, as long as you have the correct rotor radius measurement.

Q5: What's a typical g-force for common protocols?
A: Common ranges are 200-400g for cell pelleting, 10,000-20,000g for organelles, and up to 100,000g for macromolecules.